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Abstract Molecular recognition of biomolecules by single-walled carbon nanotubes can be achieved by predictably attaching a recognition site to a functionalized subunit of a guest molecule and a second functionalized subunit of a host nanotube. We introduce a molecular recognition strategy that is based on the sequence-specific photocleavage of DNA by benzoic esters. Our approach exploits the sequence specificity of fluorescence resonance energy transfer between the DNA fluorophores and the nanotube-bound quenchers and is based on the need for a low-energy excitation wavelength to avoid indiscriminate excitation of the fluorophores. This is achieved using a simplified fluorescence-detection scheme that exploits the sequence specificity of the photocleavage reaction to locate fluorophores. This strategy was used to locate cyanine dyes on a single-walled carbon nanotube. Cyanine dyes are a versatile dye family with absorption bands in both the near-infrared and visible regions of the electromagnetic spectrum, including in the visible emission wavelength region of carbon nanotubes. The manuscript discusses the synthesis of single-walled carbon nanotube (SWNT) conjugates with a CdSe quantum dot (QD) via microwave-assisted method. The conjugation process of water-soluble CdSe QDs (hydrophilic CdSe QDs) is optimized by controlling the thermal treatment. The comparative study is done by varying the reaction parameters such as the reaction time, CdSe particles concentration and solvent types. According to the experimental data, the optimum reaction conditions are obtained using 2.5 ml of solution in 2.0 mmol concentration of all materials. The prepared highly fluorescent quantum dot-nanotube conjugates (QD-SWNTs) are characterized by scanning transmission electron microscope (STEM), Fourier transform infrared spectroscopy (FT-IR), UV-vis spectroscopy, fluorescence spectrometry, and photoluminescence spectrometry. A comparison of the prepared materials with typical fluorophores shows that both target nanotubes and CdSe QDs become more colorful by conjugation and the conjugation process successfully reduces the aggregation of CdSe QDs. The manuscript highlights the synthesize carboxylated polystyrene (PS) particles to obtain stable and reactive acid-functionalized polystyrene particles via drug precipitation technique (DPT). d2c66b5586
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